Journal: Biosensors

Article Title: From the Clinic, to the Clinic: Improving the Fluorescent Imaging Quality of ICG via Amphiphilic NIR-IIa AIE Probe

doi: 10.3390/bios16020090

Figure Lengend Snippet: Chemical structure and physical and biochemical properties of TCP. ( a ) Synthetic route and chemical structure of TCP. ( b ) Normalized absorption (Ab) and fluorescence (Fl) spectra of TCP in water. ( c ) Changes in relative emission intensity (I/I 0 ) versus the water fraction of TCP in N,N-dimethylformamide. ( d ) Changes in emission intensity for TCP and ICG in water under continuous 808 nm laser irradiation (60 min, 1 W/cm 2 ). ( e ) Bright-field (left) and NIR-II fluorescent (right) images of TCP and ICG in water (10 μmol/L) on days 1 (1st) and 7 (7th). ( f ) Dynamic light scattering (DLS) analysis of hydrodynamic diameter and transmission electron microscopy (TEM) image (scale bar: 100 nm) of TCP in water. ( g ) Viability of 293 cells after 24 h incubation with TCP at indicated concentrations.

Article Snippet: The NIR-II signal was collected under a series of Thorlabs (Newton, NJ, USA) long-pass (LP) filters upon excitation at 808 nm (30 mW/cm 2 ) with varying exposure times.

Techniques: Fluorescence, Irradiation, Transmission Assay, Electron Microscopy, Incubation

Journal: Biosensors

Article Title: From the Clinic, to the Clinic: Improving the Fluorescent Imaging Quality of ICG via Amphiphilic NIR-IIa AIE Probe

doi: 10.3390/bios16020090

Figure Lengend Snippet: NIR-II imaging of long-circulating TCP. ( a ) Schematic representing TCP’s long vascular circulation, monitored using in vivo NIR-II imaging and ex vivo blood analysis at indicated post-injection time points. ( b ) Representative in vivo NIR-II fluorescence images of TCP-treated mouse in supine position at different time points under 808 nm laser irradiation (filter: LP1300 nm, exposure time: 500 ms), scale bar = 1 cm. ( c ) Representative in vivo NIR-II fluorescence images of TCP-treated mouse in prone position at different time points under 808 nm laser irradiation (filter: LP1300 nm, exposure time: 500 ms), scale bar = 1 cm. ( d ) Time-dependent SBR quantification of average fluorescent angiography of supine TCP-treated mice at different time points (mean ± s.d., n = 3). ( e ) Time-dependent SBR quantification of average fluorescent angiography of prone TCP-treated mice at different time points (mean ± s.d., n = 3); ( f ) Ex vivo quantitative analysis of TCP in mice blood at different time points (mean ± s.d., n = 3).

Article Snippet: The NIR-II signal was collected under a series of Thorlabs (Newton, NJ, USA) long-pass (LP) filters upon excitation at 808 nm (30 mW/cm 2 ) with varying exposure times.

Techniques: Imaging, In Vivo, Ex Vivo, Injection, Fluorescence, Irradiation

Journal: Biosensors

Article Title: From the Clinic, to the Clinic: Improving the Fluorescent Imaging Quality of ICG via Amphiphilic NIR-IIa AIE Probe

doi: 10.3390/bios16020090

Figure Lengend Snippet: NIR-II imaging and SBR analysis of TCP and ICG under different filters. ( a ) Fluorescent angiography of representative mouse in supine and prone positions after intravenous injection of ICG (200 μL, 1 mmol/L in 1× PBS) under LP900 (exposure time: 5 ms) and LP1000 filters (exposure time: 10 ms), scale bar: 1 cm. ( b ) SBR of blood vessels in supine mouse shown in ( a ). ( c ) SBR of blood vessels in prone mouse shown in ( a ). ( d ) Fluorescent angiography of representative mouse in supine and prone positions after intravenous injection of TCP (200 μL, 1 mmol/L in 1× PBS) under LP900 (exposure time: 5 ms) and LP1300 filters (exposure time: 500 ms), scale bar: 1 cm. ( e ) SBR of blood vessels in supine mouse shown in ( d ); ( f ) SBR of blood vessels in prone mouse shown in ( d ). ns > 0.05, *** p < 0.001, ** p < 0.01.

Article Snippet: The NIR-II signal was collected under a series of Thorlabs (Newton, NJ, USA) long-pass (LP) filters upon excitation at 808 nm (30 mW/cm 2 ) with varying exposure times.

Techniques: Imaging, Injection

Journal: Biosensors

Article Title: From the Clinic, to the Clinic: Improving the Fluorescent Imaging Quality of ICG via Amphiphilic NIR-IIa AIE Probe

doi: 10.3390/bios16020090

Figure Lengend Snippet: Comparison of NIR-II imaging between TCP and ICG. ( a , b ) Fluorescence angiography of a representative mouse after intravenous injection of TCP (100 μL, 1 mmol/L) in prone and supine positions (filter: LP1300 nm, exposure time: 500 ms, scale bar: 1 cm). ( c – e ) Cross-sectional fluorescence intensity profiles of blood vessels at the mouse head, abdomen, and leg highlighted by the red lines in ( a1 , b1 , b2 ). ( f , g ) Fluorescence angiography of a representative mouse after intravenous injection of ICG (100 μL, 1 mmol/L) in prone and supine positions (filter: LP1000 nm, exposure time: 500 ms, scale bar: 1 cm). ( h – j ) Cross-sectional fluorescence intensity profiles of blood vessels at the mouse head, abdomen, and leg highlighted by the red lines in ( f1 , g1 , g2 ). (Green, blue, and purple boxes represent the partially enlarged details of the mouse head, abdomen, and leg, respectively; scale bar: 0.5 cm).

Article Snippet: The NIR-II signal was collected under a series of Thorlabs (Newton, NJ, USA) long-pass (LP) filters upon excitation at 808 nm (30 mW/cm 2 ) with varying exposure times.

Techniques: Comparison, Imaging, Fluorescence, Injection